P. Vijay and K. Srinivasa Rao 02 06 The advanced field of cryptography makes extensive use of bioinformatics together with other domains as one of its applications The presented cypher operates by converting messages of all typesincluding texts and audio files and imagesinto sequences consisting of singlestranded DNA A matrix of randomly generated 8x8 codon squares derived from the security key serves to encrypt series of triple codon sequences The DNA encoding process creates such a significant difficulty that attackers cannot perform frequency analysis despite preserving the original encryptiondecryption rules of traditional 5x5 Playfair An interweaving operation should be added as a step to encrypt the sequence because it enhances unpredictability Workers who used this approach gained multiple benefits against Playfair cypher modifications because their system encrypted all digital content types without text processing and integrated well for DNA steganography purposes The proposed technique shows resistant behavior against cypher attacks because the original message and cipherdata bear minimal correlation between them Copyright (c) Journal of Science Engineering Technology and Management Science. All rights reserved 2026